Xiaozhen Chen1,
Fei Xiao2,
Shanwu Dong1,
Yongli Chen1,
Jiwu Wang3 
For correspondence:- Jiwu Wang Email: qcpp59@163.com
Accepted: 30 May 2021 Published: 30 June 2021
Citation:
Chen X, Xiao F, Dong S, Chen Y, Wang J.
MiR-21 inhibits the proliferation of childhood cholesteatoma glioma cells by negatively regulating the ex
© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine the effect of MiR-21 on the proliferation of glioma cells in children with cholesteatoma, and the pathway involved.
Methods: Cholesteatoma tissues from children with cholesteatoma in Otology Department of our hospital were isolated, extracted and cultured in serum-free medium of keratinocytes. The experiment comprised three groups: negative control group, blank control group and miR-21 inhibition group. Cell cycle and cell proliferation were analyzed. mRNA and protein ex
Results: The proliferation of CK cells in miR-21 group was significantly lower than that in negative control and blank control groups (p < 0.05). The proportion of CK cells at G0/G1 phase in miR-21 inhibition group was significantly higher than those in negative control and blank control groups (p < 0.05). The protein and mRNA ex
Conclusion: Inhibition of miR-21 slows down cell proliferation to some extent, and induces apoptosis of cholesteatoma cells by inducing cell cycle arrest at G0/G1 phase via a mechanism linked to the negative regulation of ex
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